Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human, mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor γ coactivator 1α (PGC1α) and uncoupling protein1(UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.
Recombinant Human/Mouse/Rat Irisin/FNDC5 (N-6His)
£155.20 – £465.60 excluding VAT
| Size | 10µg, 50µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | Recombinant Human/Mouse/Rat Fibronectin Type III Domain-containing Protein 5 is produced by our Mammalian expression system and the target gene encoding Asp32-Glu143 is expressed with a 6His tag at the N-terminus. |
| Sequence | Asp32-Glu143 |
| Fusion Tag | N-6His |
| Accession | Q8NAU1 |
| Species | Human/Mouse/Rat |
| Expressed Host | Human Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 13.4 kDa |
| AP Mol Mass | 18-28 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Research Areas | |
| Reconstitution | Please refer to the printed manual for detailed information. |
