Fagus Antibody Services

Recombinant Human NEIL1 Protein (His Tag)

£585.60£732.00 excluding VAT

SKU FAS-030794-P Category Tag

NEIL1 is a member of DNA glycosylases. DNA glycosylases are a family homologous to the bacterial Fpg/Nei family. They play a role in base excision repair which is the mechanism by which damaged bases in DNA are removed and replaced. The first step of this process is catalyzed by DNA glycosylases. They remove the damaged nitrogenous base while leaving the sugar-phosphate backbone intact, creating an apurinic/apyrimidinic site, commonly referred to as an AP site. NEIL1 functions in base excision repair of DNA damaged by oxidation or by mutagenic agents. It acts as DNA glycosylase that recognizes and removes damaged bases. NEIL1 prefers to oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand and cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3′- and 5′-phosphates.

Size

50µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding the human NEIL1 (AAH10876.1) (Met 1-Ser 390) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.

Sequence

Met 1-Ser 390

Fusion Tag

C-His

Accession

AAH10876.1

Species

Human

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 84 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

45 kDa

AP Mol Mass

45 kDa

Formulation

Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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