Fagus Antibody Services

Recombinant Human NKG2D/CD314 Protein (His Tag)(Active)

£93.60£297.00 excluding VAT

SKU FAS-033409-P Category

NKG2-D type II integral membrane protein (NKG2D) is a type II transmembrane glycoprotein which belongs to the CD94/NKG2 family. NKG2D is expressed on natural killer (NK) cells; CD8+ alpha-beta and gamma-delta T-cells. As an activating and costimulatory receptor; it involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. It provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells; leading to cytotoxic activity. It stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx; culminating in the expression of TNF-alpha. NKG2D participates in NK cell-mediated bone marrow graft rejection and survival of NK cells. It Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA; MICB; RAET1E; RAET1G; ULBP1; ULBP2; ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.

Size

10µg, 50µg

Active Protein

Active protein

Activity

Immobilized Human NKG2D-His at 10μg/ml(100 μl/well) can bind Human MICA-Fc(Cat: PKSH032753). The ED50 of Human NKG2D-His is 19.8 ug/ml .

Protein Construction

Recombinant Human NKG2-D type II Integral Membrane Protein is produced by our Mammalian expression system and the target gene encoding Phe78-Val216 is expressed with a 6His tag at the N-terminus.

Sequence

Phe78-Val216

Fusion Tag

N-6His

Accession

P26718

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

16.9 kDa

AP Mol Mass

20-30 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Research Areas

immunology

Reconstitution

Please refer to the printed manual for detailed information.

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