NKG2-D type II integral membrane protein (NKG2D) is a type II transmembrane glycoprotein which belongs to the CD94/NKG2 family. NKG2D is expressed on natural killer (NK) cells; CD8+ alpha-beta and gamma-delta T-cells. As an activating and costimulatory receptor; it involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. It provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells; leading to cytotoxic activity. It stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx; culminating in the expression of TNF-alpha. NKG2D participates in NK cell-mediated bone marrow graft rejection and survival of NK cells. It Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA; MICB; RAET1E; RAET1G; ULBP1; ULBP2; ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.
Recombinant Human NKG2D/CD314 Protein (His Tag)(Active)
£93.60 – £297.00 excluding VAT
| Size | 10µg, 50µg |
|---|---|
| Active Protein | Active protein |
| Activity | Immobilized Human NKG2D-His at 10μg/ml(100 μl/well) can bind Human MICA-Fc(Cat: PKSH032753). The ED50 of Human NKG2D-His is 19.8 ug/ml . |
| Protein Construction | Recombinant Human NKG2-D type II Integral Membrane Protein is produced by our Mammalian expression system and the target gene encoding Phe78-Val216 is expressed with a 6His tag at the N-terminus. |
| Sequence | Phe78-Val216 |
| Fusion Tag | N-6His |
| Accession | P26718 |
| Species | Human |
| Expressed Host | Human Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 16.9 kDa |
| AP Mol Mass | 20-30 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
| Research Areas | immunology |
| Reconstitution | Please refer to the printed manual for detailed information. |
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