Fagus Antibody Services

Recombinant Human Noggin/NOG Protein (aa 28-232, Fc Tag)(Active)

£72.00£262.00 excluding VAT

SKU FAS-033577-P Category Tag

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mature Human Noggin contains an N-terminal acidic region; a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates; such that mature human Noggin shares 99%; 99%; 98%; 97% and 89% aa sequence identity with mouse; rat bovine; equine and chicken Noggin; respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung; skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions; addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state; or alternatively; to differentiate into dopaminergic neurons.

Size

10µg, 50µg

Active Protein

Active protein

Activity

Immobilized Human BMP-2(Cat: PKSR030454) at 2μg/ml(100 μl/well) can bind Human Noggin-Fc. The ED50 of Human Noggin-Fc is 0.045μg/mL.

Protein Construction

Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encodingGln28-Cys232 is expressed with a Fc tag at the C-terminus.

Sequence

Gln28-Cys232

Fusion Tag

C-Fc

Accession

Q13253

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

50.2 kDa

AP Mol Mass

60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Research Areas

Developmental Biology, Stem cells

Reconstitution

Please refer to the printed manual for detailed information.

Related Product