OX40 (CD134) and its binding partner, OX40L (CD252), are members of the tumor necrosis factor receptor/tumor necrosis factor superfamily, is known to break an existing state of tolerance in malignancies, leading to a reactivation of antitumor immunity. The interaction between OX40 and OX40L plays an important role in antigen-specific T-cell expansion and survival. OX40 and OX40L also regulate cytokine production from T cells, antigen-presenting cells, natural killer cells, and natural killer T cells, and modulate cytokine receptor signaling. In line with these important modulatory functions, OX40-OX40L interactions have been found to play a central role in the development of multiple inflammatory and autoimmune diseases, making them attractive candidates for intervention in the clinic. Conversely, stimulating OX40 has shown it to be a candidate for therapeutic immunization strategies for cancer and infectious disease.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets Co-stimulatory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Recombinant Human OX40/TNFRSF4 Protein (His Tag)(Active)
£585.60 – £5,875.00 excluding VAT
| Size | 100µg, 1mg |
|---|---|
| Active Protein | Active protein |
| Activity | Measured by its binding ability in a functional ELISA.Immobilized human TNFRSF4-his at 2 μg/mL (100 μl/well) can bind human TNFSF4/mFc, The EC50 of human TNFSF4/mFc is 29 ng/mL. |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Ala 216) of human TNFRSF4 (NP_003318.1) was expressed, fused with a polyhistidine tag at the C-terminus. |
| Sequence | Met 1-Ala 216 |
| Fusion Tag | C-His |
| Accession | NP_003318.1 |
| Species | Human |
| Expressed Host | HEK293 Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg of the protein as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 21.7 kDa |
| AP Mol Mass | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 |
| Research Areas | immunology, Stem cells |
| Reconstitution | Please refer to the printed manual for detailed information. |
