CD274; also known as B7-H1 or programmed death ligand 1 (PD-L1); is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1; CD274; B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells; PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly; it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Recombinant Human PD-L1/B7-H1/CD274 Protein (His Tag)(Active)
£39.20 – £148.00 excluding VAT
Size | 10µg, 50µg |
---|---|
Active Protein | Active protein |
Activity | Immobilized Human PD-1-Fc(Cat: PKSM041289) at 10μg/ml(100 μl/well) can bind Human PD-L1-His. |
Protein Construction | Recombinant Human PD-L1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a 6His tag at the C-terminus. |
Sequence | Phe19-Thr239 |
Fusion Tag | C-His |
Accession | Q9NZQ7 |
Species | Human |
Expressed Host | Human Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 26.33 kDa |
AP Mol Mass | 34 kDa |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4. |
Research Areas | Cancer, immunology |
Reconstitution | Please refer to the printed manual for detailed information. |
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