Fagus Antibody Services

Recombinant Human PD-L1/B7-H1/CD274 Protein (His Tag)(Active)

£39.20£148.00 excluding VAT

CD274; also known as B7-H1 or programmed death ligand 1 (PD-L1); is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1; CD274; B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells; PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly; it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Size

10µg, 50µg

Active Protein

Active protein

Activity

Immobilized Human PD-1-Fc(Cat: PKSM041289) at 10μg/ml(100 μl/well) can bind Human PD-L1-His.

Protein Construction

Recombinant Human PD-L1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a 6His tag at the C-terminus.

Sequence

Phe19-Thr239

Fusion Tag

C-His

Accession

Q9NZQ7

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 90 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

26.33 kDa

AP Mol Mass

34 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4.

Research Areas

Cancer, immunology

Reconstitution

Please refer to the printed manual for detailed information.

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