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Recombinant Human PDGFRB/CD140b Protein (His Tag)(Active)

£536.00 – £4,372.00 excluding VAT

SKU FAS-031557-P Category

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Size

100µg, 1mg
Active Protein

Active protein
Activity

Measured by its binding ability in a functional ELISA. Immobilized PDGFRβ-His at 10 μg/ml (100 μl/well) can bind Cynomolgus PDGFB, The EC50 of Cynomolgus PDGFB is 3-8 ng/ml.
Protein Construction

A DNA sequence encoding the human PDGFRβ (NP_002600.1) extracellular domain (Met 1-Lys 531) was expressed, with a C-terminal polyhistidine tag.
Sequence

Met 1-Lys 531
Fusion Tag

C-His
Accession

NP_002600.1
Species

Human
Expressed Host

HEK293 Cells
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 90 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per µg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

57.6 kDa
AP Mol Mass

95-100 kDa
Formulation

Lyophilized from sterile PBS, pH 7.4
Research Areas

Signal Transduction, Cardiovascular, Cancer
Reconstitution

Please refer to the printed manual for detailed information.

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