Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. At low PH conditions, Pepsinogen C undergoes conversion into active enzyme. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.
Recombinant Human Pepsinogen C/PGC Protein (HEK293 Cells, His Tag)
£489.60 – £612.00 excluding VAT
| Size | 20µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | A DNA sequence encoding the human PGC (NP_002621.1) (Met 1-Ala 388) was fused with a polyhistidine tag at the C-terminus. |
| Sequence | Met 1-Ala 388 |
| Fusion Tag | C-His |
| Accession | NP_002621.1 |
| Species | Human |
| Expressed Host | HEK293 Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 42 kDa |
| AP Mol Mass | 42 kDa |
| Formulation | Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.5 |
| Research Areas | Signal Transduction, Microbiology, Cancer, metabolism |
| Reconstitution | Please refer to the printed manual for detailed information. |
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