Fagus Antibody Services

Recombinant Human PGA4/Pepsinogen A Protein (Fc Tag)(Active)

£576.80£721.00 excluding VAT

SKU FAS-030742-P Category Tag

PGA4 (Pepsinogen 4; group I); or Pepsinogen A; is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen; pepsinogen; whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed; and does not digest the body’s own proteins in the stomach’s lining. Five types of zymogens of pepsins; gastric digestive proteinases; are known: pepsinogens A; B; and F; progastricsin; and prochymosin. There are two major groups of pepsinogen; namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin); and each frequently has isozymogens. The PGA3; PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.

Size

50µg

Active Protein

Active protein

Activity

Measured by its ability to cleave the fluorogenic peptide substrate; Mca-RPKPVE-Nval-WRK(Dnp)-NH2; AnaSpec; Catalog # 27114.The specific activity is > 3000 pmoles/min/μg.(Activation description: The enzyme achieves its activity under acidic pH)

Protein Construction

A DNA sequence encoding the human PGA4 (NP_001073276.1) (Met 1-Ala 388) was fused with the Fc region of human IgG1 at the C-terminus.

Sequence

Met 1-Ala 388

Fusion Tag

C-Fc

Accession

P00790

Species

Human

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 80 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

67.3 kDa

AP Mol Mass

65-70 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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