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Recombinant Human PLA2G7/Lp-PLA2 Protein (His Tag)(Active)

£576.80 – £721.00 excluding VAT

SKU FAS-031390-P Category

Platelet-activating factor acetylhydrolase; also known as 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycero-phosphocholine esterase; Group-VIIA phospholipase A2; LDL-associated phospholipase A2; PAF 2-acylhydrolase; PLA2G7 and PAFAH; is secreted protein which belongs to the AB hydrolase superfamily and Lipase family. PLA2G7 / PAFAH modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. It has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. PLA2G7 / PAFAH is a potent pro- and anti-inflammatory molecule that has been implicated in multiple inflammatory disease processes; including cardiovascular disease. PLA2G7 also represents an important; potentially functional candidate in the pathophysiology of coronary artery disease (CAD). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PLA2G7 deficiency). It is a trait which is present in 27% of Japanese. It could have a significant physiologic effect in the presence of inflammatory bodily responses.

Size

50µg
Active Protein

Active protein
Activity

Measured by its ability to cleave a colorimetric peptide substrate; 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2-Thio-PAF); in the presence of 5; 5’Dithiobis(2-nitrobenzoic acid) (DTNB).The specific activity is >5000 pmoles/min/μg.
Protein Construction

A DNA sequence encoding the human PLA2G7 (Q13093-1) precursor (Met 1-Asn 441) was expressed; with a C-terminal polyhistidine tag.
Sequence

Met 1-Asn 441
Fusion Tag

C-His
Accession

Q13093-1
Species

Human
Expressed Host

HEK293 Cells
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 88 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per µg of the protein as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

49.2 kDa
AP Mol Mass

50-55 kDa
Formulation

Lyophilized from sterile 50mM NaAc, 150mM NaCl, 10% glycerol, pH 5.0
Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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