Fagus Antibody Services

Recombinant Human PPP3R1 Protein (His Tag)(Active)

£684.00£855.00 excluding VAT

PPP3R1 belongs to the calcineurin regulatory subunit family. It is a regulatory subunit of calcineurin. Calcineurin is composed of two subunits: calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation; nuclear translocation; and early gene expression in T-cells. PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. PPP3R1 is involved in protein dephosphorylation; NFAT protein import into nucleus (ortholog) and epithelial to mesenchymal transition (ortholog). It participates in calcineurin signaling pathway; mitogen activated protein kinase signaling pathway. PPP3R1 interacts with (+)-pilocarpine; 2;4-dinitrotoluene and ammonium chloride. It contains four EF-hand domains and four functional calcium-binding sites. PPP3R1 play an improtant role in the T cell activation pathway.

Size

100µg

Active Protein

Active protein

Activity

Using the Octet RED System; the affinity constant (Kd) of human PPP3R1-His bound to Human PPIA-His was 6 nM.

Protein Construction

A DNA sequence encoding the human PPP3R1 (P63098) (Gly2-Val170) was fused with a polyhistide tag at the N-terminus.

Sequence

Gly2-Val170

Fusion Tag

N-His

Accession

P63098

Species

Human

Expressed Host

Baculovirus-Insect Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 87 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

21.4 kDa

AP Mol Mass

20 kDa

Formulation

Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol, 2mM DTT

Research Areas

Signal Transduction, immunology

Reconstitution

Please refer to the printed manual for detailed information.

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