Fagus Antibody Services

Recombinant Human SerpinB3/SCCA1 Protein (His Tag)(Active)

£693.60£867.00 excluding VAT

SERPINB3, also known as SCCA-1, belongs to the serpin family. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. SERPINB3 is expressed in some hepatocellular carcinoma (at protein level). Its expression is closely related to cellular differentiation in both normal and malignant squamous cells. It seems to also be secreted in plasma by cancerous cells but at a low level. SERPINB3 significantly attenuates apoptosis by contrasting cytochrome c release from the mitochondria and by antichemotactic effect for NK cells. It may act as a protease inhibitor to modulate the host immune response against tumor cells and may be involved in the malignant behavior of squamous cell carcinoma cells.

Size

50µg

Active Protein

Active protein

Activity

Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenice peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 is < 5 nM.

Protein Construction

A DNA sequence encoding the human SERPINB3 (AAB20405.1) (Asn 2-Pro 390) was expressed, with a polyhistidine tag at the N-terminus.

Sequence

Asn 2-Pro 390

Fusion Tag

N-His

Accession

AAB20405.1

Species

Human

Expressed Host

Baculovirus-Insect Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 97 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg of the protein as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

46.7 kDa

AP Mol Mass

43 kDa

Formulation

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 3mM DTT

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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