Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.
Recombinant Human Siglec-10 (C-mFc)
£104.80 – £314.40 excluding VAT
Size | 10µg, 50µg |
---|---|
Active Protein | Active protein |
Activity | Immobilized Anti-Human Siglec10 mAb(Cat#NC035) at 2μg/ml (100 μl/well) can bind Human Siglec-10-mFc(Cat#C01G ). The ED50 of Human Siglec-10-mFc(Cat#C01G ) is 18.16 ng/ml. |
Protein Construction | Recombinant Human Sialic Acid-binding Ig-like Lectin 10 is produced by our Mammalian expression system and the target gene encoding Met17Thr546 is expressed with a mFc tag at the C-terminus. |
Sequence | Met17-Thr546 |
Fusion Tag | C-mFc |
Accession | Q96LC7 |
Species | Human |
Expressed Host | Human Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 84.6 kDa |
AP Mol Mass | 110-120 kDa |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Research Areas | Tags & Cell Markers |
Reconstitution | Please refer to the printed manual for detailed information. |
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