Fagus Antibody Services

Recombinant Human Thyroid peroxidase/TPO Protein (257 Ser/Ala, 725 Pro/Thr, His Tag)

£809.60£1,012.00 excluding VAT

SKU FAS-030705-P Category Tag

Thyroid peroxidase is a membrane-bound glycoprotein which belongs to the peroxidase family, XPO subfamily. It contains 1 EGF-like domain and 1 Sushi (CCP/SCR) domain. Thyroid Peroxidase represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. It used to be taken as the formerly so-called `microsomal antigen` several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx 100 kDa molecular weight. Thyroid peroxidase is an enzyme expressed abundantly in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine or triiodothyronine, thyroid hormones. Thyroid peroxidase plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin to form precursors of the thyroid hormones T4 and T3.

Size

100µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding the human TPO (P07202-1) extracellular domain (Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr) was fused with a polyhistidine tag at the C-terminus.

Sequence

Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr

Fusion Tag

C-His

Accession

P07202-1

Species

Human

Expressed Host

Baculovirus-Insect Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

93.8 kDa

AP Mol Mass

90-100 kDa

Formulation

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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