TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Recombinant Human Tie2/CD202b Protein (His & GST Tag)(Active)
£388.80 – £486.00 excluding VAT
| Size | 50µg |
|---|---|
| Active Protein | Active protein |
| Activity | 1.No Kinase Activity.2.Measured by its binding ability in a functional ELISA. Immobilized human TEK (aa 771-1124) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 0.31-20 μg/ml. |
| Protein Construction | A DNA sequence encoding the human TEK (NP_000450) (Gln771-Ala1124) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Sequence | Gln771-Ala1124 |
| Fusion Tag | N-His & GST |
| Accession | NP_000450 |
| Species | Human |
| Expressed Host | Baculovirus-Insect Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 92 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 68.3 kDa |
| AP Mol Mass | 64 kDa |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly |
| Research Areas | |
| Reconstitution | Please refer to the printed manual for detailed information. |
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