Fagus Antibody Services

Recombinant Human TNFRSF1B/CD120b Protein (His Tag)(Active)

£585.60£5,875.00 excluding VAT

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Size

100µg, 1mg

Active Protein

Active protein

Activity

Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-0.8 μg/ml in the presence of 0.25 ng/mL recombinant human TNF-α.

Protein Construction

A DNA sequence encoding the extracellular domain (Met 1-Asp 257) of human TNFRII (NP_001057.1) was expressed, fused with a C-terminal polyhistidine tag.

Sequence

Met 1-Asp 257

Fusion Tag

C-His

Accession

NP_001057.1

Species

Human

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 97 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

26.7 kDa

AP Mol Mass

42-45 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas

Signal Transduction, Cell biology, Cardiovascular, Neuroscience, Cancer, immunology

Reconstitution

Please refer to the printed manual for detailed information.

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