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Recombinant Human TRAIL Protein (Active)

£200.00 – £730.00 excluding VAT

SKU FAS-033422-P Category Tag

Human TNFSF10 is a type II transmembrane protein with an intracellular N-terminus and a ‘TNF homology domain’ (THD) at the extracellular C terminus. TNFSF10 can interact with several distinct receptors. Two of these receptors that belongs to TNFR superfamily, DR4 (TRAIL-R1) and DR5 (TRAIL-R2/TRICK2), are plasma membrane proteins containing intracellular death domains essential for activating apoptosis. TNFSF10 is promising for cancer therapy because it is cytotoxic and activates apoptosis in the majority of malignant cells, but not in normal cells.

Size

20µg, 100µg
Active Protein

Active protein
Activity

Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is 10.4-15.4 ng/mL.
Protein Construction

Recombinant Human TNF-Related Apoptosis-Inducing Ligand is produced by our E.coli expression system and the target gene encoding Arg115-Gly281 is expressed.
Sequence

Arg115-Gly281
Fusion Tag
Accession

NP_003801.1
Species

Human
Expressed Host

E.coli
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 95 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per µg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

19.5 kDa
AP Mol Mass

16 kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.5
Research Areas

Cell biology, Cardiovascular, Cancer
Reconstitution

Please refer to the printed manual for detailed information.

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