Fagus Antibody Services

Recombinant Human Transferrin Receptor/TFRC Protein (His Tag)

£78.40£262.00 excluding VAT

Transferrin receptor protein 1 (TFRC) belongs to the peptidase M28 family that is synthesized as a 172 amino acid (aa). TFRC regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3′-UTR. It binds one transferrin or HFE molecule per subunit and binds the HLA class II histocompatibility antigen, DR1. It Interacts with SH3BP3 and STEAP3, facilitates TFRC endocytosis in erythroid precursor cells. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. It positively regulates T and B cell proliferation through iron uptake.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Human TFRC is produced by our Mammalian expression system and the target gene encoding Leu101-Phe760 is expressed with a 6His tag at the N-terminus.

Sequence

Leu101-Phe760

Fusion Tag

N-His

Accession

P02786

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 90 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

74.9 kDa

AP Mol Mass

80-90 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH7.4.

Research Areas

Cardiovascular, Cancer, immunology, metabolism, Stem cells

Reconstitution

Please refer to the printed manual for detailed information.

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