Fagus Antibody Services

Recombinant Human UBE2A Protein (GST & His Tag)

£148.80£239.00 excluding VAT

Ubiquitin-Conjugating Enzyme E2 (UBE2A) is a member of the E2 Ubiquitin-Conjugating Enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is required for postreplication repair of UV-damaged DNA. UBE2A Interacts with RAD18 and WAC.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Human Ubiquitin-Conjugating Enzyme E2 A is produced by our E.coli expression system and the target gene encoding Met1-Cys152 is expressed with a GST; 6His tag at the N-terminus.

Sequence

Met 1-Cys152

Fusion Tag

N-GST&6His

Accession

P49459

Species

Human

Expressed Host

E.coli

Shipping

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity

> 80 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass

49.0 kDa

AP Mol Mass

52 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.

Research Areas

Cell biology, epigenetics and nuclear signal

Reconstitution

Not Applicable

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