Fagus Antibody Services

Recombinant Human UBE2A Protein (His Tag)

£536.00£670.00 excluding VAT

Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A); encoded by human DNA repair genes HHR6A; belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6 / UBC2 gene; a member of the family of ubiquitin-conjugating enzymes. In vivo; HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition; with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity; while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is consisdered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family. 

Size

100µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding the mature form of human UBE2A (P49459) (Met 1-Cys 152) was expressed; with a polyhistidine tag at the N-terminus.

Sequence

Met 1-Cys 152

Fusion Tag

N-His

Accession

P49459

Species

Human

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 80 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

19.2 kDa

AP Mol Mass

18.5 kDa

Formulation

Lyophilized from sterile PBS, 20% glycerol, pH 7.5

Research Areas

Cell biology, epigenetics and nuclear signal

Reconstitution

Please refer to the printed manual for detailed information.

Related Product