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Recombinant Human UBE2D1 Protein (GST Tag)

£24.80 – £105.00 excluding VAT

SKU FAS-033367-P Category

Ubiquitin-conjugating enzyme E2 D1（UBE2D1）belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. This enzyme is closely related to a stimulator of iron transport (SFT); and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Size

10µg, 50µg
Active Protein
Activity
Protein Construction

Recombinant Human Ubiquitin-conjugating enzyme E2 D1 is produced by our E.coli expression system and the target gene encoding Met1-Met147 is expressed with a GST tag at the N-terminus.
Sequence

Met 1-Met 147
Fusion Tag

N-GST
Accession

P51668
Species

Human
Expressed Host

E.coli
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 90 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per µg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

42.9 kDa
AP Mol Mass

25&50 kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerin, pH7.5.
Research Areas

Cell biology, epigenetics and nuclear signal
Reconstitution

Please refer to the printed manual for detailed information.

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