Fagus Antibody Services

Recombinant Human UBE2I/UBC9 Protein

£752.00£940.00 excluding VAT

UBE2I is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 



Active Protein
Protein Construction

A DNA sequence encoding the human UBE2I (P63279) (Met 1-Ser 158) was expressed and purified; with additional two amino acids (Gly & Pro) at the N-terminus.


Met 1-Ser 158

Fusion Tag




Expressed Host



This product is provided as lyophilized powder which is shipped with ice packs.


> 90 % as determined by reducing SDS-PAGE.


Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass
AP Mol Mass

18 kDa


Lyophilized from sterile PBS, 10% glycerol, 2mM DTT, pH 7.4

Research Areas

Cell biology, epigenetics and nuclear signal, metabolism


Please refer to the printed manual for detailed information.

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