Fagus Antibody Services

Recombinant Human UBE2W Protein (His Tag)

£536.00£670.00 excluding VAT

SKU FAS-030788-P Category Tag

Ubiquitin-conjugating enzymes, also known as UBE2W, E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step of protein ubiquitination. The modification of protein with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2W is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It also catalyzes monoubiquitination and "Lys-11"-linked polyubiquitination. UBE2W is also considered to regulate FANCD2 monoubiquitination. UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL). Over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradiation-induced but not MMC-induced FANCD2 monoubiquitination.

Size

100µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding the human UBE2W (Q96B02-12) (Met 1-Cys 151) was expressed, with a polyhistidine tag at the N-terminus.

Sequence

Met 1-Cys 151

Fusion Tag

N-His

Accession

Q96B02-12

Species

Human

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 97 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

19.2 kDa

AP Mol Mass

18 kDa

Formulation

Lyophilized from sterile 20mM Tris, 100mM Arg.0.1% Brij35, pH 8.5

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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