Fagus Antibody Services

Recombinant Human USP5/ISOT Protein (His Tag)

£536.00£670.00 excluding VAT

SKU FAS-030782-P Category Tag

Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored ‘Lys-48’-linked polyubiquitin disassembly. It binds linear and ‘Lys-63’-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Size

100µg

Active Protein
Activity
Protein Construction

A DNA sequence encoding the human USP5 isoform short (P45974-2) (Met 1-Ser 835) was fused with a polyhistidine tag at the C-terminus.

Sequence

Met 1-Ser 835

Fusion Tag

C-His

Accession

P45974-2

Species

Human

Expressed Host

Baculovirus-Insect Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 92 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

94.7 kDa

AP Mol Mass

100 kDa

Formulation

Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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