Fagus Antibody Services

Recombinant Human VCAM1 Protein (His Tag)

£49.60£178.00 excluding VAT

VCAM-1 is a single-pass type I membrane protein, contains 7 Ig-like C2-type domains. It is an endothelial ligand for very late antigen-4 (VLA-4) and α4?7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an “endothelial cell gate” through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Human Vascular Cell Adhesion Protein 1 is produced by our Mammalian expression system and the target gene encoding Phe25-Glu698 is expressed with a 6His tag at the C-terminus.

Sequence

Phe25-Glu698

Fusion Tag

C-6His

Accession

NP_001069.1

Species

Human

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

75.3 kDa

AP Mol Mass

86 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 2mM CaCl2, 2mM MgCl2, 5% Threhalose, pH 7.2.

Research Areas

Signal Transduction, Cardiovascular, Neuroscience, Cancer, Stem cells

Reconstitution

Please refer to the printed manual for detailed information.

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