Vanin-1 is a cell membrane protein which contains one CN hydrolase domain and belongs to the CN hydrolase family and BTD/VNN subfamily. Vanin-1 is also a member of the Vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. Vanin-1 is widely expressed with higher expression in spleen, kidney and blood and overexpressed in lesional psoriatic skin. No biotinidase activity has been demonstrated for any of the vanin proteins; however, they possess pantetheinase activity, which may play a role in oxidative-stress response. Vanin-1 is an epithelial pantetheinase that provides cysteamine to tissue and regulates response to stress. Vanin-1 is expressed by enterocytes, and its absence limits intestinal epithelial cell production of proinflammatory signals. Vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions. The early impact of vanin-1 deficiency on tumor induction was directly correlated to the amount of inflammation and subsequent epithelial proliferation rather than cell death rate. Vanin-1 molecule was shown to be involved in the control of thymus reconstitution following sub-lethal irradiation.
Recombinant Human VNN1/Vanin-1 Protein (His Tag)
£103.20 – £375.00 excluding VAT
| Size | 10µg, 50µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | Recombinant Human Vascular Non-Inflammatory Molecule 1 is produced by our Mammalian expression system and the target gene encoding Gln22-Ser490 is expressed with a 6His tag at the C-terminus. |
| Sequence | Gln22-Ser490 |
| Fusion Tag | C-6His |
| Accession | O95497 |
| Species | Human |
| Expressed Host | Human Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 53.3 kDa |
| AP Mol Mass | 83 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Research Areas | Signal Transduction, Cell biology, Cardiovascular, metabolism, Stem cells |
| Reconstitution | Please refer to the printed manual for detailed information. |
