VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members; thereby playing a role in various processes such as cell metabolism; cell polarity; apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells; such as those in testis; thymus; fetal liver; and carcinomas. As a serine/threonine kinase; VRK1 phosphorylates ‘Thr-18’ of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1); also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem; central and peripheral motor dysfunction from birth; gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.
Recombinant Human VRK1 Protein (His & GST Tag)
£388.80 – £486.00 excluding VAT
| Size | 50µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | A DNA sequence encoding the human VRK1 (Q99986) (Met 1-Lys 396) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Sequence | Met 1-Lys 396 |
| Fusion Tag | N-His & GST |
| Accession | Q99986 |
| Species | Human |
| Expressed Host | Baculovirus-Insect Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 88 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 73 kDa |
| AP Mol Mass | 65-70 kDa |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly |
| Research Areas | Signal Transduction, Cell biology, epigenetics and nuclear signal |
| Reconstitution | Please refer to the printed manual for detailed information. |
