ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interphase cells. ZWINT interacts ZW10, MIS12 and NDC80 subunit of the NDC80 complex specifically during mitosis. ZWINT is a part of the MIS12 complex which is required for kinetochore formation and spindle checkpoint activity. In addition, ZWINT is required to target ZW10 to the kinetochore at prometaphase.
Recombinant Human ZWINT Protein (His Tag)
£142.40 – £523.00 excluding VAT
Size | 10µg, 50µg |
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Active Protein | |
Activity | |
Protein Construction | Recombinant Human ZW10 Interactor is produced by our E.coli expression system and the target gene encoding Met1-Pro277 is expressed with a 6His tag at the N-terminus. |
Sequence | Met 1-Pro277 |
Fusion Tag | N-6His |
Accession | O95229 |
Species | Human |
Expressed Host | E.coli |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per µg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 33.4 kDa |
AP Mol Mass | 36 kDa |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Research Areas | Cell biology, epigenetics and nuclear signal |
Reconstitution | Please refer to the printed manual for detailed information. |