Fagus Antibody Services

Recombinant Human ZWINT Protein (His Tag)

£142.40£523.00 excluding VAT

ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interphase cells. ZWINT interacts ZW10, MIS12 and NDC80 subunit of the NDC80 complex specifically during mitosis. ZWINT is a part of the MIS12 complex which is required for kinetochore formation and spindle checkpoint activity. In addition, ZWINT is required to target ZW10 to the kinetochore at prometaphase.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Human ZW10 Interactor is produced by our E.coli expression system and the target gene encoding Met1-Pro277 is expressed with a 6His tag at the N-terminus.

Sequence

Met 1-Pro277

Fusion Tag

N-6His

Accession

O95229

Species

Human

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

33.4 kDa

AP Mol Mass

36 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Research Areas

Cell biology, epigenetics and nuclear signal

Reconstitution

Please refer to the printed manual for detailed information.

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