Fagus Antibody Services

Recombinant Klebsiella pneumoniae NEO Protein

£220.00£420.00 excluding VAT

Aminoglycoside 3′-phosphotransferase (APH(3′)), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3′-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3′) is primarily found in certain species of gram-positive bacteria.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Klebsiella pneumoniae NEO is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed.

Sequence

Met1-Phe264

Fusion Tag
Accession

P00552

Species

Klebsiella pneumoniae

Expressed Host

E.coli

Shipping

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass

29 kDa

AP Mol Mass

28-30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Research Areas
Reconstitution

Not Applicable

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