Fagus Antibody Services

Recombinant Mouse β-NGF/NGFB Protein (aa 130-239)(Active)

£49.60£103.20 excluding VAT

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.

Size

10µg, 50µg

Active Protein

Active protein

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.5-1.5 ng/ml.

Protein Construction

Recombinant Mouse beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Met130-Arg239 is expressed. 

Sequence

Met130-Arg239

Fusion Tag
Accession

P01139

Species

Mouse

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

12.4 kDa

AP Mol Mass

12 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH8.0.

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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