Fagus Antibody Services

Recombinant Mouse Carbonic Anhydrase 14/Car14 Protein (His Tag)

£210.40£391.20 excluding VAT

Mouse Ca14,also known as Carbonic anhydrase 14,is a member of large family of zinc metalloenzymes .It could catalyze reversible hydration of carbon dioxide. The reaction is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Fifteen CA isoforms have been reported so far. They have different patterns of tissue-specific expression and physiologic roles. Some CAs may serve as markers for tumors and hypoxia. CA XIV is a polypeptide consisting of an extracellular N-terminal catalytic domain, a membrane-spanning segment and a short intracellular C- terminal segment with several potential phosphorylation sites. A subset of CAs lack CA activity due to point mutations but retain esterase function. CA14 is widely expressed in the central nervous system

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Mouse Carbonic anhydrase 14 is produced by our E.coli expression system and the target gene encoding Ala16-Met290 is expressed with a 6His tag at the N-terminus.

Sequence

Ala16-Met290

Fusion Tag

N-6His

Accession

Q9WVT6

Species

Mouse

Expressed Host

E.coli

Shipping

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass

32.3 kDa

AP Mol Mass

40 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0.

Research Areas
Reconstitution

Not Applicable

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