Fagus Antibody Services

Recombinant Mouse CD16-2/FCGR4 Protein (His&AVI Tag), Biotinylated(Active)

£588.00£1,350.40 excluding VAT

Fcgr4, also known as CD16-2, is one of the receptors for Fc region of IgG which involve in immune responses. Fcgr4 mainly functions in cellular response to lipopolysaccharide, NK T cell proliferation, regulation of sensory perception of pain, wound healing etc. Three groups are included for Fc γ receptors (FcR), and they are Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). Among these, CD64 possess high affinity even for monomeric IgG, while CD32 and CD16 display a relative lower affinity for IgG. Genes encodes these receptors are diverse differing by species and cell types. The aggregation of FcR having immunoreceptor tyrosine-based activation motifs (ITAMs) activates sequentially src family tyrosine kinases and syk family tyrosine kinases that connect transduced signals to common activation pathways shared with other receptors. FcR with ITAMs elicit cell activation, endocytosis, and phagocytosis. Fcgr4 belongs to Fc γ RIII (CD16) group.


20µg, 100µg

Active Protein

Active protein


1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA.2. Labeling ratio of biotin to protein: 0.6

Protein Construction

The amino acid sequence corronding to (Met 1-Gln 203) of mouse FCGR4 (NP_653142.2) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.


Met 1-Gln 203

Fusion Tag

C-His-AVI, Biotinylated





Expressed Host

HEK293 Cells


This product is provided as lyophilized powder which is shipped with ice packs.


> 95 % as determined by reducing SDS-PAGE.


< 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

24.2 kDa

AP Mol Mass

Lyophilized from sterile PBS, pH 7.4

Research Areas

Please refer to the printed manual for detailed information.

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