Fagus Antibody Services

Recombinant Mouse CD86/B7-2 Protein (His Tag)

£49.60£142.40 excluding VAT

T-lymphocyte activation antigen CD86 (B7-2) is a glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. Mouse CD86 shares 59% and 81% aa sequence identity with human and rat CD86, respectively. It contains 1 Ig-like C2-type domainand 1 Ig-like V-type domain. It is highly expressed on activated antigen presenting cells. CD86 involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. It may play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. It is expressed by activated B-lymphocytes and monocytes and promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Mouse CD86 is produced by our Mammalian expression system and the target gene encoding Val24-Lys244 is expressed with a 6His tag at the C-terminus.

Sequence

Val24-Lys244

Fusion Tag

C-His

Accession

P42082

Species

Mouse

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

25.9 kDa

AP Mol Mass

35-85 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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