Fagus Antibody Services

Recombinant Mouse CSNK2A1/CK2A1 Protein (His & GST Tag)(Active)

£573.60 excluding VAT

SKU FAS-040295-P Category Tag

Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha’) in a homomeric or heteromeric conformation. Whilst the alpha- and alpha’-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha’-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via th phosphorylation of Akt.

Size

50µg

Active Protein

Active protein

Activity

The specific activity was determined to be 34 nmol/min/mg using casein as substrate.

Protein Construction

A DNA sequence encoding the mouse CSNK2A1 (Q60737) (Met1-Gln391) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sequence

Met1-Gln391

Fusion Tag

N-His-GST

Accession

Q60737

Species

Mouse

Expressed Host

Baculovirus-Insect Cells

Shipping

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity

> 85 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass

72.9 kDa

AP Mol Mass

65 kDa

Formulation

Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly

Research Areas
Reconstitution

Not Applicable

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