Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha’) in a homomeric or heteromeric conformation. Whilst the alpha- and alpha’-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha’-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via th phosphorylation of Akt.
Recombinant Mouse CSNK2A1/CK2A1 Protein (His & GST Tag)(Active)
£573.60 excluding VAT
Size | 50µg |
---|---|
Active Protein | Active protein |
Activity | The specific activity was determined to be 34 nmol/min/mg using casein as substrate. |
Protein Construction | A DNA sequence encoding the mouse CSNK2A1 (Q60737) (Met1-Gln391) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Sequence | Met1-Gln391 |
Fusion Tag | N-His-GST |
Accession | Q60737 |
Species | Mouse |
Expressed Host | Baculovirus-Insect Cells |
Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. |
Purity | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Stability and Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
Mol Mass | 72.9 kDa |
AP Mol Mass | 65 kDa |
Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly |
Research Areas | |
Reconstitution | Not Applicable |
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