Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. follistatin are expressed and subserve local regulatory roles in numerous extragonadal tissues, including brain, adrenal, bone marrow, and placenta but perhaps most notably in anterior pituitary-the classical target tissue for inhibin, the activin-follistatin system may play a key role in early embryogenesis. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone follistatin is a binding protein to activin. Since activin binds to follistatin, it is imperative to determine the nature of the activin/follistatin binding complex.
Recombinant Mouse Follistatin/FST (FS288) Protein (Fc Tag)(Active)
£585.60 excluding VAT
| Size | 50µg |
|---|---|
| Active Protein | Active protein |
| Activity | Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED50 for this effect is typically 40-200 ng/mL in the presence of 10 ng/mL rhActivin A. |
| Protein Construction | A DNA sequence encoding the mouse FST (P47931) (Met 1-Asn 317) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Sequence | Met 1-Asn 317 |
| Fusion Tag | C-Fc |
| Accession | P47931 |
| Species | Mouse |
| Expressed Host | CHO Stable Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 58.4 kDa |
| AP Mol Mass | 66 kDa |
| Formulation | Lyophilized from sterile PBS, pH 7.4 |
| Research Areas | |
| Reconstitution | Please refer to the printed manual for detailed information. |
