Fagus Antibody Services

Recombinant Mouse Galectin-1/LGALS1 Protein (Active)

£224.80£451.20 excluding VAT

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.

Size

20µg, 100µg

Active Protein

Active protein

Activity

Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 1-5 μg/ml.

Protein Construction

A DNA sequence encoding the mouse Lgals1 (P16045) (Met 1-Glu 135) was expressed and purified.

Sequence

Met 1-Glu 135

Fusion Tag
Accession

P16045

Species

Mouse

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

15 kDa

AP Mol Mass

15 kDa

Formulation

Lyophilized from sterile PBS, 100mM β-Lactose, pH 7.4

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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