Growth hormone receptor, also known as GH receptor and GHR, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and type 1 subfamily. GHR contains one fibronectin type-III domain. Growth hormone receptor / GHR is expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 of GHR is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression of GHR in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 of GHR is expressed in lung, stomach and muscle. Growth hormone receptor / GHR is a receptor for pituitary gland growth hormone. It is involved in regulating postnatal body growth. On ligand binding, it couples to the JAK2 / STAT5 pathway. Isoform 2 of GHR up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Defects in GHR are a cause of Laron syndrome (LARS) which is a severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may also be a cause of idiopathic short stature autosomal (ISSA) which is defined by a subnormal rate of growth.
Recombinant Mouse GHR/GHBP Protein (His & Fc Tag)(Active)
£585.60 excluding VAT
Size | 100µg |
---|---|
Active Protein | Active protein |
Activity | Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 0.5-2 μg/mL in the presence of 50 ng/mL human growth hormone. |
Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Gln 273) of mouse GHR (NP_034414.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. |
Sequence | Met 1-Gln 273 |
Fusion Tag | C-His-Fc |
Accession | NP_034414.2 |
Species | Mouse |
Expressed Host | HEK293 Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 56.8 kDa |
AP Mol Mass | 70-80 kDa |
Formulation | Lyophilized from sterile PBS, pH 7.4 |
Research Areas | |
Reconstitution | Please refer to the printed manual for detailed information. |
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