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Recombinant Mouse Glypican-1/GPC1 Protein (His Tag)(Active)

£93.60 – £237.60 excluding VAT

SKU FAS-041030-P Category Tag

Glypican-1 is a cell membrane protein and belongs to the glypican family. The protein may act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. It is required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

Size

10µg, 50µg
Active Protein

Active protein
Activity

Immobilized Human FGFb(Cat: PKSH032437) at 2μg/ml(100 μl/well) can bind Mouse GPC1-His. The ED50 of Mouse GPC1-His is 786 ng/ml .
Protein Construction

Recombinant Mouse Glypican-1 is produced by our Mammalian expression system and the target gene encoding Asp24-Ser529 is expressed with a 6His tag at the C-terminus.
Sequence

Asp24-Ser529
Fusion Tag

C-6His
Accession

Q9QZF2
Species

Mouse
Expressed Host

Human Cells
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 90 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per μg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

56.7 kDa
AP Mol Mass

56kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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