From: £178.40
Granzyme B(GZMB) contains 1 peptidase S1 domain and belongs to the peptidase S1 family. This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp and seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. The protein cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
| Size | 10µg, 50µg |
|---|---|
| Active Protein | |
| Activity | |
| Protein Construction | Recombinant Mouse Granzyme B is produced by our Mammalian expression system and the target gene encoding Gly19-Ser247 is expressed with a 6His tag at the C-terminus. |
| Sequence | Gly19-Ser247 |
| Fusion Tag | C-6His |
| Accession | P04187 |
| Species | Mouse |
| Expressed Host | Human Cells |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. |
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg as determined by the LAL method. |
| Stability and Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Mol Mass | 26.5 kDa |
| AP Mol Mass | 35kDa |
| Formulation | Supplied as a 0.2 μm filtered solution of PBS, pH7.4. |
| Research Areas | |
| Reconstitution | Not Applicable |
