Fagus Antibody Services

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Recombinant Mouse IL-1A/IL-1α Protein

£200.00£584.00 excluding VAT

SKU FAS-041080-P Category Tag

Mouse Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.

Size

20µg, 100µg

Active Protein

Active protein

Activity

Measure by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is 2 x 108 IU/mg.

Protein Construction

Recombinant Mouse Interleukin-1 alpha is produced by our E.coli expression system and the target gene encoding Ser115-Ser270 is expressed.

Sequence

Ser115-Ser270

Fusion Tag
Accession

P01582

Species

Mouse

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

18.0 kDa

AP Mol Mass

16 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of 50mM TrisHCl, 200mM NaCl, pH 8.0.

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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