Mouse Interleukin 33 (IL-33) is a 30 kDa proinflammatory cytokine which may also regulates gene transcription in producer cells. IL-33 is constitutively expressed in smooth muscle and airway epithelia. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1. BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and also leads to severe pathological changes in mucosal organs.
Recombinant Mouse Interleukin-33/IL-33 Protein (Active)
£200.00 – £584.00 excluding VAT
| Size | 20µg, 100µg |
|---|---|
| Active Protein | Active protein |
| Activity | Measure by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is 2 x 107 IU/mg. |
| Protein Construction | Recombinant Mouse Interleukin-33 is produced by our E.coli expression system and the target gene encoding Ser109-Ile266 is expressed. |
| Sequence | Ser109-Ile266 |
| Fusion Tag | |
| Accession | Q8BVZ5 |
| Species | Mouse |
| Expressed Host | E.coli |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 17.6 kDa |
| AP Mol Mass | 18 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4. |
| Research Areas | |
| Reconstitution | Please refer to the printed manual for detailed information. |
