Fagus Antibody Services

Recombinant Mouse MBL1 Protein (His Tag)(Active)

£585.60 excluding VAT

SKU FAS-040943-P Category Tag

Mannose-binding lectin (MBL), also named mannose or mannan-binding protein (MBP), is a C-type lectin which participates in the innate immune system as an activator of the complement system and as opsonin after binding to certain carbohydrate structures on microorganisms and pathogens. Its function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL recognizes carbohydrate patterns found on the surface of a large number of pathogenic micro-organisms including bacteria, viruses, protozoa and fungi. Binding of MBL to a micro-organism results in activation of the lectin pathway of the complement system. Two forms of MBL, MBL-A and MBL-C, were characterized in rodents, rabbits, bovine and rhesus monkeys, whereas only one form was identified in humans, chimpanzees and chickens. The two forms are encoded by two distinct genes named MBL1 and MBL2, which have been identified in many species including the pig. The MBL1 and MBL2 genes encode mannan-binding lectins (MBL) A and C, respectively, that are collagenous lectins (collectins) produced mainly by the liver. The MBL1 gene encodes MBL-A, which has bacteria-binding properties in pigs and rodents but is mutated to a pseudogene in humans and chimpanzees. Deficiency of MBL is probably the most common human immunodeficiency and is associated with an increased risk of mucosally acquired infections including meningococcal disease. MBL could modify disease susceptibility by modulating macrophage interactions with mucosal organisms at the site of initial acquisition.

Size

100µg

Active Protein

Active protein

Activity

Using the Octet RED System, the affinity constant (Kd) of mouse MBL bound to biotinylated mannan was 72nM.

Protein Construction

A DNA sequence encoding the mature form of mouse MBL (NP_034905.1) (Ser 18-Ala 239) was expressed with a N-terminal polyhistidine tag.

Sequence

Ser 18-Ala 239

Fusion Tag

N-His

Accession

NP_034905.1

Species

Mouse

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 90 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

25.8 kDa

AP Mol Mass

32 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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