Programmed Death-1 (PD-1), firstly cloned from mouse T cell hybridoma 2B4.11, is one member of CD28/CTLA-4 superfamily. PD-1 belongs to type I transmembrane protein and acts as an important immunosuppressive molecule. This family also include members of CD28, CTLA-4 and ICOS.The mouse Programmed Death-1 protein, encoded by PD-1 gene, comprises four parts including a putative 20 aa signal peptide, a 149 aa extracellular region, a 21 aa transmembrane domain and a 98 aa cytoplasmic region. The cytoplamsic tail of PD-1 contains two structural motifs, an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM) formed by two tyrosine residues which make the difference in PD-1 signal mediating. Mouse PD-1 is expressed in thymus and shares about 69% aa sequence identity with human PD-1. Recently, programmed death-1 (PD-1) with its ligands, programmed death ligand B7H1 (PD-L1) and B7DC (PD-L2), was found to regulate T-cell activation and tolerance, upon ligand binding, inhibiting T-cell effector functions in an antigen-specific manner. PD-1 gene knocked out mice would induce some autoimmune diseases, which suggests that PD-1 acts as a co-inhibitory molecule actively participating in maintaining peripheral tolerance. Thus, PD-1 may be a useful target for the immunologic therapy of carcinoma,infection,autoimmune diseases as well as organ transplantation.
Recombinant Mouse PD-1/PDCD1 Protein (Fc Tag)(Active)
£49.60 – £133.60 excluding VAT
| Size | 10µg, 50µg |
|---|---|
| Active Protein | Active protein |
| Activity | Immobilized Human PD-L1-His(Cat: PKSH033557) at 2μg/ml(100 μl/well) can bind Mouse PD-1-Fc. |
| Protein Construction | Recombinant Mouse PD-1 is produced by our Mammalian expression system and the target gene encoding Leu25-Gln167 is expressed with a Fc tag at the C-terminus. |
| Sequence | Leu25-Gln167 |
| Fusion Tag | C-Fc |
| Accession | Q02242 |
| Species | Mouse |
| Expressed Host | Human Cells |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg as determined by the LAL method. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 43.3 kDa |
| AP Mol Mass | 58-85 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0. |
| Research Areas | |
| Reconstitution | Please refer to the printed manual for detailed information. |
Related Product
Related products
-
-
-
Protein
Recombinant MERS-CoV S-trimer Protein (R751S, C-6His)(Active)
£304.00 – £5,875.00 excluding VAT -
-
Protein
Recombinant ZIKV (strain Zika SPH2016) Stem/anchor domain of flavivirus envelope glycoprotein E protein (Fc Tag)
£540.00 – £855.00 excluding VAT -
-
-
-
-