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Recombinant Mouse SCF/c-Kit Ligand Protein (Active)

£142.40 – £262.40 excluding VAT

SKU FAS-041151-P Category Tag

Mouse stem cell factor (SCF), is the ligand for the receptor-type protein-tyrosine kinase KIT. It plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. It also promotes phosphorylation of PIK3R1, which is the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5.

Size

10µg, 50µg
Active Protein

Active protein
Activity

Measured by the dose-dependent stimulation of TF-1 cells. The ED50 for this effect is 4-12 ng/mll.
Protein Construction

Recombinant Mouse Stem Cell Factor is produced by our E.coli expression system and the target gene encoding Lys26-Ala189 is expressed.
Sequence

Lys26-Ala189
Fusion Tag
Accession

P20826
Species

Mouse
Expressed Host

E.coli
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 95 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per μg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

18.4 kDa
AP Mol Mass

16 kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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