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Recombinant Mouse Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)

From: £105.60

SKU FAS-041394-P Category

Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts

Size

10µg, 50µg
Active Protein

Active protein
Activity

Immobilized Mouse Siglec-15-His (Cat#PKSM041394) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 27.7 ng/ml.
Protein Construction

Recombinant Mouse Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Arg24-Thr262 is expressed with a 6His tag at the C-terminus.
Sequence

Arg24-Thr262
Fusion Tag

C-6His
Accession

A7E1W8
Species

Mouse
Expressed Host

Human Cells
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 95 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per µg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

26.5 kDa
AP Mol Mass

30-40 kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, 150mM NaCl, 5% Thehalose, 0.3% Chaps, pH 7.4.
Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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