T cell immunoreceptor with Ig and ITIM domains (TIGIT), also called WUCAM, VSIG9 and Vstm3, is a member of the CD28 family within the Ig superfamily of proteins. TIGIT contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM), and is expressed on regulatory, memory, activated T cells and NK cells. TIGIT binds to CD155(PVR) that appear on dendritic cells (DC), macrophages and endothelium with high affinity, and CD112(PVRL2) with lower affinity, but not CD113 (PVRL3). TIGIT-Fc fusion protein could interact with PVR on DC and enhance the secretion of IL-10, but inhibit the macrophage activation. Mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges, while knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses.
Recombinant Mouse TIGIT Protein (Fc Tag)
£93.60 – £237.60 excluding VAT
Size | 10µg, 50µg |
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Active Protein | |
Activity | |
Protein Construction | Recombinant Mouse TIGIT is produced by our Mammalian expression system and the target gene encoding Gly26 – Thr143 is expressed with a Fc tag at the C-terminus. |
Sequence | Gly26-Thr143 |
Fusion Tag | C-Fc |
Accession | NP_001139797 |
Species | Mouse |
Expressed Host | Human Cells |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg as determined by the LAL method. |
Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Mol Mass | 40.1 kDa |
AP Mol Mass | 50-60 kDa |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Research Areas | |
Reconstitution | Please refer to the printed manual for detailed information. |