Tissue?Factor, also known as thromboplastin and CD142,?belongs to the tissue factor family which conteins 294 amino acid (aa). Mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). It is a single-pass type I membrane protein that expressed in the liver oscillates in a circadian manner. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Recombinant Mouse Tissue factor is produced by our Mammalian expression system and the target gene encoding Ala29-Glu251 is expressed with a 6His tag at the C-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
> 95 % as determined by reducing SDS-PAGE.
< 1.0 EU per μg as determined by the LAL method.
|Stability and Storage||
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|AP Mol Mass||
Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, 0.05%Brij35, pH7.5.
Please refer to the printed manual for detailed information.