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Recombinant Mouse TNF-alpha/TNFA Protein (aa 80-235)(Active)

From: £200.00

SKU FAS-041240-P Category

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. TNF-alpha Protein & AntibodyImmune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Size

20µg, 100µg

Active Protein

Active protein

Activity

Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is 2.5x 107 IU/mg.

Protein Construction

A DNA sequence encoding the soluble form of mouse TNF-α (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the C-terminus.

Sequence

Leu 80-Leu 235

Fusion Tag
Accession

NP_038721.1

Species

Mouse

Expressed Host

E.coli

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 98 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

17 kDa

AP Mol Mass

17 kDa

Formulation

Lyophilized from sterile 50mM Tris 0.2M NaCl, pH 7.2

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.