Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. TNF-alpha Protein & AntibodyImmune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Recombinant Mouse TNF-alpha/TNFA Protein (aa 80-235)(Active)
£200.00 – £584.00 excluding VAT
| Size | 20µg, 100µg |
|---|---|
| Active Protein | Active protein |
| Activity | Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is 2.5x 107 IU/mg. |
| Protein Construction | A DNA sequence encoding the soluble form of mouse TNF-α (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the C-terminus. |
| Sequence | Leu 80-Leu 235 |
| Fusion Tag | |
| Accession | NP_038721.1 |
| Species | Mouse |
| Expressed Host | E.coli |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 17 kDa |
| AP Mol Mass | 17 kDa |
| Formulation | Lyophilized from sterile 50mM Tris 0.2M NaCl, pH 7.2 |
| Research Areas | |
| Reconstitution | Please refer to the printed manual for detailed information. |
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