Fagus Antibody Services

Linkedin Twitter
Linkedin Twitter

Recombinant Mouse TRAIL R2/TNFRSF10B Protein (Fc Tag)(Active)

£93.60 – £237.60 excluding VAT

SKU FAS-041271-P Category

Mouse tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is a member of the TNFR family which contains 1 death domain and 3 TNFR-Cys repeats. TNFRSF10B exhibits high structural and functional homology to TRAIL-R1 (DR-4). TNFRSF10B is highly expressed in heart, lung, lymphocytes, spleen and kidney. In addition, it is regulated by the tumor suppressor p53. TNFRSF10B is the receptor for the cytotoxic ligand TNFSF10/TRAIL. It promotes the activation of NF-kappa-B and is essential for ER stress-induced apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases mediating apoptosis.

Size

10µg, 50µg
Active Protein

Active protein
Activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 92.04 ng/ml in the presence of 40 ng/mL of TNFSF10 .
Protein Construction

Recombinant Mouse TRAILR2 is produced by our Mammalian expression system and the target gene encoding Asn53-Ser177 is expressed with a Fc tag at the C-terminus.
Sequence

Asn53-Ser177
Fusion Tag

C-Fc
Accession

Q9QZM4
Species

Mouse
Expressed Host

Human Cells
Shipping

This product is provided as lyophilized powder which is shipped with ice packs.
Purity

> 95 % as determined by reducing SDS-PAGE.
Endotoxin

< 1.0 EU per μg as determined by the LAL method.
Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass

40.9 kDa
AP Mol Mass

50-75 kDa
Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

Related Product

Related products

All Products