Fagus Antibody Services

Recombinant Rat CD172a/SIRPA Protein (His Tag)(Active)

£585.60£684.00 excluding VAT

SKU FAS-030273-P Category Tag

Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.Immune CheckpointImmune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Size

100µg

Active Protein

Active protein

Activity

1.Measured by its binding ability in a functional ELISA. 2.Immobilized rat SIRPA-His at 10μg/mL (100μL/well) can bind biotinylated rat CD47-His, the EC50 of biotinylated rat CD47-His is 0.1-0.4ug/mL.

Protein Construction

A DNA sequence encoding the rat SIRPA (P97710) (Met1-Asn373) was expressed with a polyhistidine tag at the C-terminus.

Sequence

Met1-Asn373

Fusion Tag

C-His

Accession

P97710

Species

Rat

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

39 kDa

AP Mol Mass

63-80 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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